ncRNA name | GNAQ siRNA |
ncRNA Category | siRNA |
Disease name | uveal melanoma (UM) |
Species | Homo sapiens |
Tissues/Cell_line | primary choroidal UM cell line: Mel202, Mel270, Mel290, 92.1 and OCM1A; liver metastasis cell line from Mel270: Omm1.3 |
Methods | CCK-8 assay, flow cytometry, colony assay, apoptosis assay, transfection, immunoblot |
Expression pattern | associated |
Functional description | Downregulation of GNAQ decreased cell viability of the mutant cells. Induction of autophagy in combination-treated GNAQ-mutant cells was accompanied by an increase in pAMPK, LC3 and beclin1. This was confirmed in mutant cells after GNAQ depletion by siRNA. |
PubMed ID | 23443802 |
Year | 2013 |
Title | Inhibition of mutant GNAQ signaling in uveal melanoma induces AMPK-dependent autophagic cell death. |
Drug-related ncRNA | NO |
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